mercoledì 25 marzo 2020

CORONAVIRUS NO PLOT BUT ESCAPE FROM A CHINESE LABORATORY- we generated and characterized a chimeric virus expressing the peak of bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone. OTHER FRENCH AND DUTCH GUILTERS ABSOLUTE IN THE USA

Being an expert in Social Political Marketing and with a great ability to imagine documents on the Internet, there is a list of the method that led me with a good percentage of chances of being reliable:

I would say 60/70%

Let's start from the Sars problem to understand what could have happened:

Bats have been identified as a natural reservoir for an increasing number of emerging zoonotic viruses, including henipaviruses and variants of rabies viruses. Recently, we and another group independently identified several species of horseshoe bats (genus Rhinolophus) as the host of the reservoir for a large number of viruses that have a close genetic relationship with the coronavirus associated with severe acute respiratory syndrome. (SARS).

The discovery of bat-type SARS coronaviruses and the large genetic diversity of coronaviruses in bats shed new light on the origin and transmission of SARS coronaviruses.

Severe acute respiratory syndrome (SARS) represents the first pandemic of the 21st century of a communicable disease with a previously unknown cause. The pandemic began in November 2002 and was brought under control in July 2003, after spreading to 33 countries on 5 continents, causing> 8,000 infections and> 700 deaths (1). The outbreaks were caused by a recently emerged coronavirus, now known as SARS coronavirus (SARS-CoV).

Epidemiological studies indicated that the first cases of patients were more likely than subsequent cases to report living near a product market but not near a farm, and almost half of them were food managers with likely contact with animals.

The presence of SARs similar to SARS had been reported in several species of horseshoe bats in the genus Rhinolophus. We discovered, in a study on horseshoe bat species in different regions of the People's Republic of China in 2004 These results indicate independent species crossing events. They also indicate that a SARS epidemic may recur in the future.

The recent discovery of a group of different SARs similar to SARs in bats supports the possibility of these events and further underlines the need to understand the distribution and transmission of reservoirs to prevent future outbreaks.


PRACTICALLY FOLLOW ME IN THIS OFFICIAL SCENARIO WITH SCIENTIFIC DATA PUBLISHED ON FAMOUS SCIENTIFIC MAGAZINES.

So the main problem was for researchers in this branch and for China, an evolved nation that has the epicenter of the phenomenon, to find remedies, vaccines to be able to face the possible spread of a Coronavirus such as COVID-19 in the future through studies and changes made to RNA and using bats and mice.

The discovery of SARS-CoV has enhanced the search for new coronaviruses of human and animal origin.

Bats were chosen as the main target due to the diversity of their species, the large population, the vast geographical distribution, the long-distance migration capacity and the habit of roosting in large groups.

In practice, Research starts on bats in search of methodology to combat a possible future infection with Coronavirus, modifying SARS RNA.

Here the problem arises!

And what are the laboratories in the world that carry out this research? Many but in particular I have rediscovered the supply chain between

HOLLAND, FRANCE AND CHINA

A Dutch laboratory starts with a research on these elements, and comes into contact with the Military and Scientific laboratory of Wuan built with French technoclogy

And what do you do in this laboratory? several studies and not just one in order not to build biological weapons but to prevent a possible spread of the Coronavirus Virus.

It starts with these procedures:

The viruses used in this study were isolated from bat brain homogenates by direct intracranial inoculation (IC) of 3-day-old suckling mice.

The studies are funded by:

This work was supported by grants from the Ministry of Science and Technology, People's Republic of China

 China Centers for Disease Control and Prevention (CDC)
AND THIS IS THE DISCOVERY:


The bat-like SARS virus shows the potential to infect humans, according to the results of the study


By HELEN BRANSWELL @ HelenBranswell9 NOVEMBER 2015

Researchers from the University of North Carolina at Chapel Hill said that a virus belonging to the same family as SARS - severe acute respiratory syndrome - appears to be capable of infecting human respiratory tract cells. The discovery was a surprise because the team thought the virus would have to go through a process known as adaptation, which means it should have acquired the ability to infect human cells by first learning how to infect another mammal's cells.

Researchers from the University of North Carolina at Chapel Hill said that a virus belonging to the same family as SARS - severe acute respiratory syndrome - appears to be capable of infecting human respiratory tract cells. The discovery was a surprise because the team thought that the virus would have to go through a process known as adaptation, which means it would have to acquire the ability to infect human cells by first learning how to infect another mammal's cells.

Attention this is not the moment of the spread of COVID-19 here we are still at the time of the evolution from SARS in Mers, but it is sufficient to understand that the studies should be addressed in that sector.

UNC scientists wanted to see if cousin viruses, the coronaviruses carried by Chinese horseshoe bats, posed a threat to people. They used one, SHC014, as the group representative.

They inserted a key part of the virus, its peak protein, into a SARS virus and then conducted experiments to see if the hybrid virus could infect human respiratory tract cells (in a dish) and mice vulnerable to the SARS virus.
lead author Vineet Menachery, who researches viral immunology, in an interview with STAT. "Based on what was known in the literature, we would have expected that the viruses that came out of bats would need that million-dollar mutation."

 Another coronavirus expert, dr. Stanley Perlman of the University of Iowa suggested that the document was a useful investigation. But he noted that the hybrid virus had been attenuated - weakened - and said that the virus would probably have had to adapt more to people before it could spread widely.

SARS was not a highly transmissible virus.

And being able to do something within the artificial confines of a laboratory does not guarantee that it will happen in nature. For a bat virus to start infecting people, the bat should make contact with people in order to allow transmission.

Even if only one person becomes infected, the virus should work efficiently in human cells, producing many copies of itself that could be coughed up or injected into other people's airways. "There are many steps along this road," said Menachery. “SHC014 has taken a step forward. But there are still many other factors involved. "

He and his co-authors noted that they had to stop part of their work because of the policies of the United States government.

The United States has a moratorium on so-called function-gain research, which includes some research that increases the ability of a pathogen like a virus to infect people or spread among them.

It is important to understand the potential that these viruses can infect people, said Fouchier, who works at the Erasmus Medical Center in Rotterdam, especially in light of ongoing outbreaks involving the coronavirus known as MERS, or Middle Eastern respiratory syndrome.

AT THIS POINT WE HAVE THE PROOF THAT THE UNITED STATES PUT THE MORATORIUM OUT OF THE SCENARIO AND THEY HAVE BEEN VERY GOOD, BUT HERE COME INTO PLAY THE DUTCH LABORATORY THAT, THANKS TO THE FINANCING OF THE NETHERLANDS, PROCEEDS WITH THE SEARCH FOR HAVING ALL OF THE .

The emergence of severe acute respiratory syndrome Coronavirus (SARS-CoV) and Middle Eastern respiratory syndrome (MERS) -CoV underlines the threat of transmission events between species leading to outbreaks in humans.

Using the SARS-CoV infectious clone, we generated and characterized a chimeric virus that expresses the bat coronavirus peak SHC014 in a mouse-adapted SARS-CoV backbone.

The results indicate that group 2b viruses encoding the SHC014 peak in a wild-type backbone can efficiently use multiple ACE2 receptor orthologists, replicate effectively in primary cells of the human airway and obtain in vitro titers equivalent to epidemic strains. by SARS-CoV. In addition, in vivo experiments demonstrate the replication of the chimeric virus in the mouse lung with significant pathogenesis.

AND THESE IDIOTS WHAT DO THE SOLUTION DISCOVER? NO, ABSOLUTELY BUT ONLY THAT THIS CHANGE IN THE LABORATORY CAN BE MORE VIRULENT AND PROPAGATE DIRECTLY FROM A MAN'S BAT AND THAT THE POSSIBILITIES OF CARE ARE ZERO.

Evaluation of SARS-based immunotherapy and prophylactic modalities revealed poor efficacy; both approaches with monoclonal antibodies and vaccines have failed to neutralize and protect against VOCs using the new spike protein. Importantly, based on these results, we have synthetically redistributed an infectious full-length recombinant SHC014 virus and demonstrated solid viral replication both in vitro and in vivo. Together, the work highlights a continuing risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.

SARS and MERS emerged in 2003 and 2012 respectively and caused a worldwide pandemic that caused thousands of lives, while SADS hit the pig industry in 2017.

THEREFORE WE HAVE THESE DISEASES AND THE LABORATORY CHIMERA THAT CAN GENERATE A NEW #CORONAVIRUS HIGHLY MODIFIED COUNTER AND THAT CAN EASILY PASS FROM ANIMAL TO BAT.
The only thing that the studies say assuming that the virus by mistake did not run away from the research laboratory to find solutions and not by bacteriological war is what they understood in 2015.

To predict the next CoV that will cause a virus outbreak in the future, we list the general factors that may contribute to this outbreak. First, bats are home to a large number of highly diversified VOCs. CoV genomes are known to undergo regular recombinations during infection and a rich genetic heritage can facilitate this process.

Secondly, bat species are widely distributed and live close to humans. Third, viruses are pathogenic and transmissible.

In this context, the SADS-CoV and SARS-CoV epidemics in China are not unexpected.

With this model, there are other VOCs that have not yet caused virus outbreaks but which should be monitored.

Two bat-born VOCs have caused large-scale epidemics in China for fourteen years, highlighting the risk of a future bat VOC outbreak in this nation.

In this review, we have summarized the current results relating to the epidemiology of bat CoV in China, in order to explore associations between CoV species, bat species and geographic locations, and finally we aim to predict the transmission potential between species of these Bat CoV.

Here we examine the disease potential of a SARS-like virus, SHC014-CoV, which is currently circulating in Chinese horseshoe bat populations 1.

HERE IS HOWEVER YOUR DECISION I HAVE IS A MODIFICATION OF THE COVID IN THE BATS OR AS I BELIEVE FROM THE WUHAN LABORATORY.

I remind you that in a previous research article I had always discovered that the staff of the Laboratory built by the French level 4 complained of being precarious as a job and of having always different staff with greater risks for the safety of the same laboratory.

HERE'S HOW IT WENT:

"Using the SARS-CoV 2 reverse genetics system, we generated and characterized a chimeric virus that expresses the bat coronavirus peak SHC014 in a mouse-adapted SARS-CoV backbone. The results indicate that the group 2b viruses that encode the SHC014 peak in a wild type backbone can efficiently use multiple SARS receptor orthologists in human angiotensin that converts enzyme II (ACE2), effectively replicates in primary cells of the human airway and obtain in vitro titres equivalent to epidemic SARS-CoV strains. In addition, in vivo experiments demonstrate the replication of the chimeric virus in the mouse lung with significant pathogenesis. The evaluation of the modalities "

AND WHAT THESE LABORATORIES AND STUDIES SUPPORT:

Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.

WHATEVER YOU GO, FORGET THE VACCINE! THEIR SAME SEARCHES SAY IT'S VERY DIFFICULT TO FIND IT.




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